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Open Access Highly Accessed Research

Water vapour and heat combine to elicit biting and biting persistence in tsetse

Charles JF Chappuis*, Steve Béguin, Michèle Vlimant and Patrick M Guerin

Author Affiliations

Institute of Biology, Laboratory of Animal Physiology, University of Neuchâtel, Rue Emile-Argand 11, 2000, Neuchâtel, Switzerland

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Parasites & Vectors 2013, 6:240  doi:10.1186/1756-3305-6-240

Published: 19 August 2013

Abstract

Background

Tsetse flies are obligatory blood feeders, accessing capillaries by piercing the skin of their hosts with the haustellum to suck blood. However, this behaviour presents a considerable risk as landing flies are exposed to predators as well as the host’s own defense reactions such as tail flicking. Achieving a successful blood meal within the shortest time span is therefore at a premium in tsetse, so feeding until replete normally lasts less than a minute. Biting in blood sucking insects is a multi-sensory response involving a range of physical and chemical stimuli. Here we investigated the role of heat and humidity emitted from host skin on the biting responses of Glossina pallidipes, which to our knowledge has not been fully studied in tsetse before.

Methods

The onset and duration of the biting response of G. pallidipes was recorded by filming movements of its haustellum in response to rapid increases in temperature and/or relative humidity (RH) following exposure of the fly to two airflows. The electrophysiological responses of hygroreceptor cells in wall-pore sensilla on the palps of G. pallidipes to drops in RH were recorded using tungsten electrodes and the ultra-structure of these sensory cells was studied by scanning and transmission electron microscopy.

Results

Both latency and proportion of tsetse biting are closely correlated to RH when accompanied by an increase of 13.1°C above ambient temperature but not for an increase of just 0.2°C. Biting persistence, as measured by the number of bites and the time spent biting, also increases with increasing RH accompanied by a 13.1°C increase in air temperature. Neurones in wall-pore sensilla on the palps respond to shifts in RH.

Conclusions

Our results show that temperature acts synergistically with humidity to increase the rapidity and frequency of the biting response in tsetse above the levels induced by increasing temperature or humidity separately. Palp sensilla housing hygroreceptor cells, described here for the first time in tsetse, are involved in the perception of differences in RH.

Keywords:
Tsetse; Glossina pallidipes; Biting behaviour; Biting fly; Blood-feeding; Hygroreception; Thermoreception; Dry cell