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Open Access Research

Pathogenic analysis of Borrelia garinii strain SZ isolated from northeastern China

Qiong Wu, Zhijie Liu, Jidong Wang, Youquan Li, Guiquan Guan, Jifei Yang, Ze Chen, Jianxun Luo* and Hong Yin*

Author Affiliations

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Key Laboratory of Grazing Animal Diseases MOA, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, China

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Parasites & Vectors 2013, 6:177  doi:10.1186/1756-3305-6-177

Published: 17 June 2013

Abstract

Background

Various genospecies of Borrelia burgdorferi sensu lato (s.l.) have been identified from patients and animals worldwide. Genospecies-related dissemination of disease has been reported. The present study aimed to elucidate the pathogenicity of infections caused by B. garinii SZ isolated in China. B. burgdorferi B31 and B. afzelii BO23 were used for comparison.

Methods

Spirochete load in blood and tissue samples of infected mice were measured by minor groove binder-based real-time polymerase chain reaction. The kinetics of spirochete dissemination and disease severity were assessed in BALB/c mice.

Results

The pattern of bacterial load differed between the three genospecies. The B. garinii SZ strain is highly pathogenic and can trigger multi-system pathological damage in mice.

Conclusions

Spirochete dissemination, persistence, tissue tropism and disease severity varied significantly, suggesting that different genospecies may play an important role in the pathogenicity and development of clinical diseases.

Keywords:
B. burgdorferi; Pathogenicity; Kinetics of spirochete; Disease severity