Table 3

Effect of silymarin with/without praziquantel on serum transforming growth factor-β1 and matrix metalloproteinase-2, 10 and 18 weeks post infection of mice with S.mansoni

Animal groups

Serum TGF-β1 (ng/ml)

Serum MMP-2 (ng/ml)


10 wks

18 wks

10 wks

18 wks


Uninfected (vehicle)

7.80 ± 0.69

7.98 ± 0.48

150.40 ± 18.17

181.00 ± 6.72


Infected (Vehicle)

†††

19.98 ± 2.37

†††

40.85 ± 1.41

††

265.43 ± 21.65

†††

374.00 ± 32.16


Infected+ PZQ

‡‡‡

11.32 ± 0.85

(43.34%)

†††‡‡‡

19.71 ± 0.24

(51.75%)

††‡

210.40 ± 8.13

(20.73%)

†††‡‡‡

226.83 ± 11.69

(39.35%)


Infected + silymarin

†††‡

13.44 ± 0.75

(32.73%)

†††

36.84 ± 1.44

(9.82%)

††

241.31 ± 36.25

(9.093%)

†††

283.80 ± 42.20

(24.12%)


Infected + PZQ + silymarin

‡‡‡§§

7.83 ± 0.65

(60.81%)

†††‡‡‡

18.29 ± 0.73

(55.23%)

‡‡§

165.33 ± 14.20

(37.71%)

‡‡‡§

187.60 ± 11.57

(49.84%)


Data are presented as mean ± SEM (n = 8 in each group).

Mice were administered praziquantel (PZQ; 500 mg/kg/day for 2 days) in the 7th week post infection (PI) and silymarin (750 mg/kg/day, 5 days/week for 6 weeks) at the 4th and 12th weeks PI for 6 weeks and were killed 10 and 18 weeks PI respectively. Numbers in parentheses indicate the percentage of reduction from infected (vehicle) group.

†† Significantly different from uninfected (vehicle) group at P < 0.01 and ††† at P < 0.001. ‡ Significantly different from infected (vehicle) group at P < 0.05, ‡‡ at P < 0.01 and ‡‡‡ at P < 0.001. § Significantly different from infected treated with PZQ group at P < 0.05 and §§ at P < 0.01.

El-Lakkany et al. Parasites & Vectors 2012 5:9   doi:10.1186/1756-3305-5-9

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