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Open Access Research

Molecular evidence of Culex pipiens form molestus and hybrids pipiens/molestus in Morocco, North Africa

Fadila Amraoui12, Mhamed Tijane2, Mhammed Sarih1 and Anna-Bella Failloux3*

Author Affiliations

1 Institut Pasteur du Maroc, Laboratoire des Maladies Vectorielles, 1 Place Louis Pasteur, Casablanca, 20360, Morocco

2 Faculté des Sciences, Laboratoire de Biochimie et Immunologie, 4 avenue Ibn Battouta, Rabat, BP 1014 RP, Morocco

3 Institut Pasteur, Department of Virology, Arboviruses and Insect Vectors, 25-28 rue du Docteur Roux, Paris, 75724, France

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Parasites & Vectors 2012, 5:83  doi:10.1186/1756-3305-5-83

Published: 27 April 2012

Abstract

Background

Culex pipiens L. is the most widespread mosquito vector in temperate regions including North Africa. Cx. pipiens has two recognized forms or biotypes; pipiens and molestus are morphologically indistinguishable with distinct behavior and physiology that may influence their vectorial status. In our study, we prospected for the different forms of Cx. pipiens in Morocco.

Methods

Cx. pipiens larvae were collected in 9 sites throughout Morocco during summer 2010 and reared until imago stage. Cx. pipiens was identified using diagnostic primers designed for the flanking region of microsatellite CQ11.

Results

We established the presence of both forms of Cx. pipiens and their hybrids in Morocco.

Conclusions

Molecular identification provides the first evidence of the presence of Cx. pipiens form molestus in Morocco and hybrids between pipiens and molestus forms in North Africa. The epidemiological implications of our findings are discussed.

Keywords:
Culex pipiens complex; Microsatellite; Molecular taxonomy; Morocco; North Africa