Table 2 |
||||
| Primers used for amplification and sequencing of gltA and GroEL genes ofN. mikurensis, and the amplification of the Msp2 gene ofA. phagocytophilum | ||||
| Gene | Name | Type | Sequence | Reference |
| gltA | NMik fo-gltA | Primer (forward) | 5’-aagtgcatgctttgctacatt-‘3 | This study |
| gltA | NMik re-gltA | Primer (reverse) | 5’-tcatgatctgcatgtaaaataaat-‘3 | This study |
| GroEL | NMikGroEL-F2a | Primer (forward) | 5’-ccttgaaaatatagcaagatcaggtag-‘3 | This study |
| GroEL | NMikGroEL-R2b | Primer (reverse) | 5’-ccaccacgtaacttatttagtactaaag -‘3 | This study |
| GroEL | NMikGroEL-P2a | Probe (RED) | 5’-RED-cctctactaattattgctgaagatgtagaaggtgaagc-BHQ2-‘3 | This study |
| GroEL | NMik fo-groEL | Primer (forward) | 5’-gaagyatagtytagtatttttgtc-‘3 | [18] |
| GroEL | NMik re-groEL | Primer (reverse) | 5’-ttaacttctacttcacttgaacc-‘3 | [18] |
| GroEL | NMik seq1groEL | Primer (reverse) | 5’-acatcacgcttcatagaaag-‘3 | [18] |
| GroEL | NMik seq2groEL | Primer (forward) | 5’-aaaggaattagtattagaatcttt-‘3 | [18] |
| GroEL | NMik seq3groEL | Primer (forward) | 5’-aatatagcaagatcaggtagac-‘3 | [18] |
| GroEL | NMik seq4groEL | Primer (reverse) | 5’-cttccattttaactgctaattc-‘3 | [18] |
| Msp2 | ApMSP2F | Primer (forward) | 5’-atggaaggtagtgttggttatggtatt-‘3 | [26] |
| Msp2 | ApMSP2R | Primer (reverse) | 5’-ttggtcttgaagcgctcgta-‘3 | [26] |
| Msp2 | ApMSP2P | Probe (FAM) | 5’-FAM-tggtgccagggttgagcttgagattg-BHQ1-‘3 | [26] |
Primers were either identical to or slightly modified from the primers described in the reference papers.
Jahfari et al. Parasites & Vectors 2012 5:74 doi:10.1186/1756-3305-5-74
