Table 1 |
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Differentiation between Leishmania subgenera by SYBR Green melting temperatures (Tm) from amplified minicircles kDNA conserved region |
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Source |
Samples |
Origin |
Melting Temperature (°C ± SD) |
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Promastigote reference strains |
L (V.) braziliensis (MHOM/BR/1975/M2903) |
IOC/FIOCRUZ |
77.34 ± 0.01 |
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L (V.) shawi (MCEB/BR/1984/M8408) |
IOC/FIOCRUZ |
77.34 ± 0.01 |
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L (V.) naiffi (MDAS/BR/1979/M5533) |
IOC/FIOCRUZ |
77.34 ± 0.01 |
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L (V.) lainsoni (MHOM/BR/1981/M6426) |
IOC/FIOCRUZ |
77.37 ± 0.01 |
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L (V.) guyanensis (MHOM/BR/1975/M4147) |
IOC/FIOCRUZ |
77.39 ± 0.01 |
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L (L.) infantum (Syn. L. chagasi) (MHOM/BR/1974/PP75) |
IOC/FIOCRUZ |
78.95 ± 0.01 |
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L (L.) amazonensis (MHOM/BR/1977/LTB0016) |
IOC/FIOCRUZ |
78.94 ± 0.00 |
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Human clinical specimens |
Cutaneous leishmaniasis (skin biopsies) |
Rio de Janeiro/RJ |
77.68 ± 0.38 |
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Visceral leishmaniasis (peripheral blood and bone marrow aspirates) |
Campo Grande/MS |
78.94 ± 0.37 |
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Field sand flies |
Lutzomyia intermedia |
Rio de Janeiro/RJ |
77.25 ± 0.15 |
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Lutzomyia migonei |
Rio de Janeiro/RJ |
77.25 ± 0.15 |
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Lutzomyia cruzi |
Corumbá/MS |
78.98 ± 0.10 |
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Lutzomyia forattini |
Corumbá/MS |
78.98 ± 0.10 |
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IOC: Oswaldo Cruz Institute, FIOCRUZ: Oswaldo Cruz Foundation, RJ: State of Rio de Janeiro, MS: State of Mato Grosso do Sul, SD: standard deviation The melting temperatures of kDNA amplified products were analyzed from samples obtained from Leishmania promastigotes reference strains, clinical specimens from cutaneous and visceral leishmaniases patients and field-captured phlebotomine insects. The dissociation curves analyses revealed distinct melting temperatures between the subgenera Viannia and Leishmania (p < 0.001 - Mann-Whitney Rank Sum Test). |
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Pita-Pereira et al. Parasites & Vectors 2012 5:15 doi:10.1186/1756-3305-5-15 |
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