Open Access Research

Development and validation of IIKC: an interactive identification key for Culicoides (Diptera: Ceratopogonidae) females from the Western Palaearctic region

Bruno Mathieu14*, Catherine Cêtre-Sossah2, Claire Garros2, David Chavernac2, Thomas Balenghien2, Simon Carpenter3, Marie-Laure Setier-Rio4, Régine Vignes-Lebbe5, Visotheary Ung5, Ermanno Candolfi1 and Jean-Claude Delécolle1

Author Affiliations

1 IPPTS, Université de Strasbourg, EA 4438, 67000, Strasbourg, France

2 Cirad, UMR Contrôle des maladies, 34398, Montpellier, France

3 Institute for Animal Health, Pirbright, GU24 0NF, United Kingdom

4 EID-Méditerranée, 34184, Montpellier, France

5 UMR 7207 CNRS-MNHN-Université Pierre et Marie Curie, 75005, Paris, France

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Parasites & Vectors 2012, 5:137  doi:10.1186/1756-3305-5-137

Published: 9 July 2012

Abstract

Background and methods

The appearance of bluetongue virus (BTV) in 2006 within northern Europe exposed a lack of expertise and resources available across this region to enable the accurate morphological identification of species of Culicoides Latreille biting midges, some of which are the major vectors of this pathogen. This work aims to organise extant Culicoides taxonomic knowledge into a database and to produce an interactive identification key for females of Culicoides in the Western Palaearctic (IIKC: Interactive identification key for Culicoides). We then validated IIKC using a trial carried out by six entomologists based in this region with variable degrees of experience in identifying Culicoides.

Results

The current version of the key includes 98 Culicoides species with 10 morphological variants, 61 descriptors and 837 pictures and schemes. Validation was carried out by six entomologists as a blind trial with two users allocated to three classes of expertise (beginner, intermediate and advanced). Slides were identified using a median of seven steps and seven minutes and user confidence in the identification varied from 60% for failed identifications to a maximum of 80% for successful ones. By user class, the beginner group successfully identified 44.6% of slides, the intermediate 56.8% and the advanced 74.3%.

Conclusions

Structured as a multi-entry key, IIKC is a powerful database for the morphological identification of female Culicoides from the Western Palaearctic region. First developed for use as an interactive identification key, it was revealed to be a powerful back-up tool for training new taxonomists and to maintain expertise level. The development of tools for arthropod involvement in pathogen transmission will allow clearer insights into the ecology and dynamics of Culicoides and in turn assist in understanding arbovirus epidemiology.

Keywords:
Multi-entry key; Identification key; Interactive key; Bluetongue; African horse sickness; Culicoides; Vectors