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Open Access Highly Accessed Research

A user-friendly software to easily count Anopheles egg batches

Ali Mollahosseini1, Marie Rossignol2, Cédric Pennetier34, Anna Cohuet2, António dos Anjos5, Fabrice Chandre2 and Hamid Reza Shahbazkia1*

Author Affiliations

1 Departamento de Engenharia Eletrónica e Informática, Universidade do Algarve, 8008-139 Faro, Portugal

2 Institut de Recherche pour le Développement, UMR MIVEGEC (UM1-UM2-CNRS 5290-IRD 224), 911 avenue Agropolis, B.P. 64501, 34394 Montpellier Cedex 5, France

3 Institut de Recherche pour le Développement, UMR MIVEGEC (UM1-UM2-CNRS 5290-IRD 224), Contonou, Benin

4 Centre de Recherche Entomologique de Cotonou, Ministry of Health, Cotonou, Benin

5 Departamento de Ciências e Tecnologias, ISMAT - Instituto Superior Manuel Teixeira Gomes, 8500-508 Portimão, Portugal

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Parasites & Vectors 2012, 5:122  doi:10.1186/1756-3305-5-122

Published: 19 June 2012

Abstract

Background

Studies on malaria vector ecology and development/evaluation of vector control strategies often require measures of mosquito life history traits. Assessing the fecundity of malaria vectors can be carried out by counting eggs laid by Anopheles females. However, manually counting the eggs is time consuming, tedious, and error prone.

Methods

In this paper we present a newly developed software for high precision automatic egg counting. The software written in the Java programming language proposes a user-friendly interface and a complete online manual. It allows the inspection of results by the operator and includes proper tools for manual corrections. The user can in fact correct any details on the acquired results by a mouse click. Time saving is significant and errors due to loss of concentration are avoided.

Results

The software was tested over 16 randomly chosen images from 2 different experiments. The results show that the proposed automatic method produces results that are close to the ground truth.

Conclusions

The proposed approaches demonstrated a very high level of robustness. The adoption of the proposed software package will save many hours of labor to the bench scientist. The software needs no particular configuration and is freely available for download on: http://w3.ualg.pt/∼hshah/eggcounter/ webcite.