Research
A dysflagellar mutant of Leishmania (Viannia) braziliensis isolated from a cutaneous leishmaniasis patient
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* Corresponding author: Silvia RB Uliana srbulian@icb.usp.br
1 Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, 05508-900, Brasil
2 Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, 74605-050, Brazil
3 Laboratório de Biologia Molecular de Parasitas e Vetores, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, 21045-900, Brazil
4 Núcleo de Medicina Tropical, Universidade Federal do Pará, Belém, 66055-240, Brazil
5 Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21944-970, Brazil
6 Universidade Federal do Rio de Janeiro, Pólo Avançado de Xerém, Rio de Janeiro, Brazil
Parasites & Vectors 2012, 5:11 doi:10.1186/1756-3305-5-11
Published: 11 January 2012Additional files
Additional file 1:
Molecular identification and phylogenetic relationships of the EFSF6 isolate. Dendrogram based on ITS1/5.8S/ITS2 sequences from the following organisms: L. (L.) braziliensis EFSF6 [GenBank JQ061322], L. (L.) chagasi [GenBank AJ000305.1], L. (L.) donovani [GenBank AJ000293.1, AM901450.1], L. (L.) major [GenBank AJ000310.1, DQ300195], L. (L.) amazonensis [GenBank AJ000314], L. (L.) mexicana [GenBank AF466383, AF466380.1], L. (V.) guyanensis [GenBank FJ753387, AJ000299.1, AJ000300.1], L. (V.) braziliensis [GenBank AJ300483, AJ300484, AJ300483] e Leptomonas mirabilis [GenBank AY180153.1]. The numbers at the branches refer to parsimony percentage bootstrap values derived from 100 replicates.
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Phase contrast imaging of L. (V.) braziliensis M2903 promastigotes in M199 medium. Representative 30 seconds video, taken at 72 h of culture started with an inoculum of 1 × 106 parasites/ml. This movie shows the general movement of typical L. (V.) braziliensis promastigotes. Note the fast moving parasites that travel across the field and the typical rosettes.
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Phase contrast imaging of L. (V.) braziliensis EFSF6 promastigotes in M199 medium. Representative 30 seconds video, taken at 72 h of culture started with an inoculum of 1 × 106 parasites/ml. This movie shows that these parasites tend to gather in groups. No fast movement is observed. A vibration is noted on the clusters of cells.
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