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Open Access Research

Assessment of exposure to Plasmodium falciparum transmission in a low endemicity area by using multiplex fluorescent microsphere-based serological assays

Jean Biram Sarr12*, Eve Orlandi-Pradines4, Sonia Fortin2, Cheikh Sow2, Sylvie Cornelie1, François Rogerie2, Soihibou Guindo2, Lassana Konate3, Thierry Fusaï4, Gilles Riveau2, Christophe Rogier45 and Franck Remoue1

Author Affiliations

1 MIVEGEC (UM1-CNRS 5290-IRD 224), Montpellier, France

2 ONG Espoir Pour La Santé (EPLS), Saint-Louis, Senegal

3 Laboratoire d'Ecologie Vectorielle et Parasitaire (LEVP), UCAD, Dakar, Senegal

4 IRBA & UMR6236, Marseille, France

5 Institut Pasteur de Madagascar, Antananarivo, Madagascar

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Parasites & Vectors 2011, 4:212  doi:10.1186/1756-3305-4-212

Published: 7 November 2011

Abstract

Background

The evaluation of malaria transmission intensity is a crucial indicator for estimating the burden of malarial disease. In this respect, entomological and parasitological methods present limitations, especially in low transmission areas. The present study used a sensitive multiplex assay to assess the exposure to Plasmodium falciparum infection in children living in an area of low endemicity. In three Senegalese villages, specific antibody (IgG) responses to 13 pre-erythrocytic P. falciparum peptides derived from Lsa1, Lsa3, Glurp, Salsa, Trap, Starp, Csp and Pf11.1 proteins were simultaneously evaluated before (June), at the peak (September) and after (December) the period of malaria transmission, in children aged from 1 to 8 years.

Results

Compared to other antigens, a high percentage of seropositivity and specific antibody levels were detected with Glurp, Salsa1, Lsa3NR2, and Lsa1J antigens. The seropositivity increased with age for all tested antigens. Specific IgG levels to Glurp, Salsa1, Lsa3NR2, and Lsa1J were significantly higher in P. falciparum infected children compared to non-infected and this increase is significantly correlated with parasite density.

Conclusion

The multiplex assay represents a useful technology for a serological assessment of rapid variations in malaria transmission intensity, especially in a context of low parasite rates. The use of such combined serological markers (i.e. Glurp, Lsa1, Lsa3, and Salsa) could offer the opportunity to examine these variations over time, and to evaluate the efficacy of integrated malaria control strategies.