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Table 3
*Serological and PCR results for eight patients infected with Bartonella koehlerae.*
Patient	Date	IFA Reciprocal Titers				PCR Results

		*B. koehlerae*	*B. henselae*	*Bvb II*	*R. rickettsii*	ITS PCR	rpoB PCR

	10-2008	64	<16	<16	<16	Bvb*^,a	Neg
1	02-2009**	<16	<16	<16	<16	B. koehlerae^a	B.koehlerae^a
1	04-2009**	<16	<16	<16	<16	Bspp^b	Neg

2	03-2009	<16	<16	32	64	B. koehlerae^c	B.koehlerae^a
2	04-2009	NA	NA	NA	NA	Neg	Neg
2	07-2009**	<16	<16	<16	16	B. koehlerae^a	Neg
2	10-2009**	<16	<16	<16	<16	Neg	Neg

3	03-2009	64	<16	64	<16	B. koehlerae^a	B.koehlerae^a
3	04-2009	NA	NA	NA	NA	B. koehlerae^c	Neg
3	05-2009**	64	<16	32	<16	Neg	Bvb*^,a
3	08-2009**	256	<16	16	<16	Neg	Neg
3	09-2009**	<16	<16	<16	<16	Neg	Neg

4	4-13-2009	<16	<16	<16	32	B. koehlerae^b	Neg
4	4-30-2009**	<16	<16	<16	<16	Neg	Bvb*^,a
4	6-02-2009**	<16	<16	32	<16	Neg	Neg
4	6-09-2009**	<16	<16	64	16	Neg	Neg
4	6-30-2009**	64	<16	64	<16	Neg	Neg

5	05-2009	NA	NA	NA	NA	B. koehlerae^a	B.koehlerae^a

6	05-2009	NA	NA	NA	NA	B. koehlerae^a	B.koehlerae^a

7	04-2009	64	<16	32	128	B. koehlerae^b	Neg
7	06-2009	64	<16	64	64	Neg	Bvb*,^a
7	07-2009	64	<16	<16	64	B. henselae^a	Neg
7	11-2009**	256	<16	<16	16	B. henselae^a	Neg

8	04-2009	64	<16	<16	16	B. koehlerae^b	Neg
8	05-2009	64	<16	<16	<16	B. koehlerae^a	B.koehlerae^a
8	07-2009	64	<16	<16	32	Neg	Neg
8	08-2009	<16	<16	32	<16	Neg	Neg

All ITS and rpoB PCR amplicons were sequenced to confirm genus and species identity. *Bvb = Bartonella vinsonii subsp. berkhoffii genotype II Bspp = amplicon generated with 16S-23 S ITS primers, but unable to obtain a DNA sequence. ** Denotes post-antibiotic testing. NA = Sample not available. ^a= amplicon sequenced from blood or serum ^b= amplicon sequenced from BAPGM enrichment blood culture ^c= amplicons sequenced from both blood and BAPGM enrichment blood culture
Breitschwerdt et al. Parasites & Vectors 2010 3:76 doi:10.1186/1756-3305-3-76

Table 3