Table 1

Mortality of V. destructor and the degree of gene knockdown under different RNAi experimental conditions.

Treatment

Application Route

Survival at 48 h (%)

VdGSTMu knockdown (%)^a

dsRNA in 0.9% NaCl

topical droplet

79 (n = 14)

0% (n = 3)

dsRNA in 0.2% Triton-X100

topical droplet

0 (n = 6)

n/d

distilled water

immersion

0 (n = 15)

n/a

0.2% Triton-X100

immersion

0 (n = 15)

n/a

0.9% NaCl

immersion

80 (n = 15)

n/a

dsRNA in 0.9% NaCl

immersion

76 (n = 21)

87 ± 0.7% (3)

dsRNA

injection

50 (n = 62)

96 ± 3.7% (4)

^a n/d, not determined; n/a, not appropriate; data presented mean ± SEM

Campbell et al. Parasites & Vectors 2010 3:73   doi:10.1186/1756-3305-3-73