Table 5

Details of selected primers and probe used to amplify Anaplasma (msp2, 16 S RNA) and Rickettsia genes (ompB,17 kDa, gltA).

Primer

Gene

Nucleotide sequences (5' to 3')

Product size (bp)

Apmsp2f

msp2

ATG-GAA-GGT-AGT-GTT-GGT-TAT-GGT-ATT

77

Apmsp2r

TAC-GAG-CGC-TTC--AAG-ACC-AA

Apmsp2p

TGG-TGC-CAG-GGT-TGA-GCT-TGA-GAT-TG

probe

GER-3

16 S RNA

TAG-ATC-CTT-CTT-AAC-GGA-AGG-GCG

151

GER-4

AAG-TGC-CCG-GCT-TAA-CCC-GCT-GGC

EE-1

16 S RNA

TCC-TGG-CTC-AGA-ACG-AAC-GCT-GGC-GGC

928

EE-2

AGT-CAC-TGA-CCC-AAC-CTT-AAA-TGG-CTG

EE-3

GTC-GAA-CGG-ATT-ATT-CTT-TAT-AGC-TTG-C

EE-4

CCC-TTC-CGT-TAA-GAA-GGA-TCT-AAT-CTC-C

OMP B-IF

omp B

CCA-ATG-GCA-GGA-CTT-AGC-TAC-T

257

OMP B-IR

AGG-CTG-GCT-GAT-ACA-CGG-AGT-AA

17 SFG-F

17 kDa

GCT-CTTGCA-ACT-TCT-ATG-TT

431

17 SFG-R

CAT-TGT-TCG-TCA-GGT-TGG-CG

SFG-CS-F

gltA

TGC-CAA-ATG-TTC-ACG-GTA-CTT-T

74

SFG-CSR

CAC-AAT-GGA-AAG-AAA-TGC-ACG-A

SFG-CS-P

TGC-AAT-AGC-AAG-AAC-CGT-AGG-CTG-GAT-G

probe

Severinsson et al. Parasites & Vectors 2010 3:66   doi:10.1186/1756-3305-3-66