Table 3

Serological, culture and molecular test results for the household pets

Bartonella IFA Reciprocal Titers

PCR/DNA Sequencing Results

Pet Designation

B. henselae

Bvb Genotype II

Bvb Genotype III

Direct Extraction

BAPGM Enrichment Culture

Subculture Isolate

JE Cat

512

128

64

Bh H1

Bh H1

Bh H1

CO Cat

<16

128

<16

Bh H1

Bh H1

Bh H1

SN Cat

256

128

64

Neg

Neg

NIO

JA Cat

2048

2048

2048

Bh H1

Bh H1

Bh H1

TO Dog

<16

<16

<16

Neg

Neg

NIO

EM Dog

<16

<16

<16

Neg

Neg

NIO

Neg = DNA was not amplified using Bartonella 16S-23S intergenic spacer (ITS) primers.

NIO = No isolate obtained by subculture following BAPGM (Bartonella alpha Proteobacteria growth medium) pre-enrichment culture.

Bh H1 = Bartonella henselae ITS Houston I like strain by DNA sequencing

Breitschwerdt et al. Parasites & Vectors 2010 3:29   doi:10.1186/1756-3305-3-29