Table 2 |
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|
PCR primers and conditions |
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| Primers |
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|
|
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| DNA region |
Forward |
Reverse |
|
|
||
| COX1 |
Schisto5' |
Schisto3' |
| TCTTTRGATCATAAGCG |
TAATGCATMGGAAAAAAACA |
|
|
|
||
| ITS |
ITSF TAACAAGGTTTCCGTAGGTGAA |
ITSR TGCTTAAGTTCAGCGGGT |
|
|
||
|
25 μl PCR reactions were carried out using Ready-to-go PCR Beads (Amersham) and 10 pmol of each primer for the DNA region to be amplified as above. Thermal cycling conditions were: 2 min denaturing at 95°C: 40 cycles of 30 s at 95°C, 30 s at 40°C, 2 min at 72°C; followed by final extension period of 7 min at 72°C. Positive amplification was checked by 0.8% Gel Red agarose gel electrophoresis of 3 μl of each amplicon, which were visualised using a UVP gel doc system (Fig 1). |
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Webster Parasites & Vectors 2009 2:50 doi:10.1186/1756-3305-2-50 |
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