Table 1 |
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|
Exposure conditions of the preserved samples and resulting PCRs produced from their gDNA extracts |
||||
| Sample sets |
Conditions |
PCR result |
||
|
|
||||
| Lab. |
Temperature°C |
Time |
COX1 |
ITS |
|
|
||||
| 1 |
26-28 |
3 days |
✓ |
✓ |
|
|
||||
| 2 |
26-28 |
1 week |
✓ |
✓ |
|
|
||||
| 3 |
26-28 |
2 weeks |
✓ |
✓ |
|
|
||||
| 4 |
37 |
3 days |
✓ |
✓ |
|
|
||||
| 5 |
37 |
1 week |
✓ |
✓ |
|
|
||||
| 6 |
37 |
2 weeks |
✓ |
✓ |
|
|
||||
| 7 |
4 |
6 months |
✓ |
✓ |
|
|
||||
| 8 |
-20 |
6 months |
✓ |
✓ |
|
|
||||
| Field |
Conditions |
COX1 |
ITS |
|
|
|
||||
| 9 |
Tropical field (28-32°C) setting for 2 weeks + transport by plane as hold luggage |
✓ |
✓ |
|
|
|
||||
| 10 |
As in 9 and then storage at 4°C for up to 6 months |
✓ |
✓ |
|
|
|
||||
| 11 |
As in 9 and then storage at -20°C for up to 6 months |
✓ |
✓ |
|
|
|
||||
|
Each sample set 1-8 included 2 of each larval stage (cercariae, miracidia) and eggs preserved in 5 μl or 10 μl of RNAlater® solution. Sample sets 9-10 contain 2 miracidia collected in the field and stored in 5 μl RNAlater®. ✓ corresponds to 100% of the samples producing a positive PCR and sequences of good quality. |
||||
|
Webster Parasites & Vectors 2009 2:50 doi:10.1186/1756-3305-2-50 |
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