Research
Transcriptome analysis of Taenia solium cysticerci using Open Reading Frame ESTs (ORESTES)
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* Corresponding author: Edmundo C Grisard grisard@ccb.ufsc.br
1 Laboratório de Neurociências (LIM27), Departamento e Instituto de Psiquiatria, Faculdade de Medicina da Universidade de São Paulo, Rua Dr. Ovídio Pires de Campos 785, CEP 05403-010, São Paulo, SP, Brazil
2 Laboratórios de Protozoologia e de Bioinformática, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina (UFSC), Caixa postal 476, CEP 88040-970, Florianópolis, SC, Brazil
3 Laboratório de Biologia Molecular e Bioinformática, Área das Ciências Biológicas e da Saúde, Universidade do Oeste de Santa Catarina (UNOESC), CEP 89600-000, Joaçaba, SC, Brazil
4 Departamento de Ciências Básicas, Faculdade de Zootecnia e Engenharia de Alimentos. Universidade de São Paulo (USP), Av. Duque de Caxias Norte, 225, Centro, CEP 13630-000, Pirassununga, SP, Brazil
5 Departamento de Biologia Molecular e Biotecnologia and Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves 9500, Prédio 4342131, Setor IV, Campus do Vale, CEP 91501-970, Porto Alegre, RS, Brazil
6 Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia (UEA), NR4 7TJ, Norwich, Norfolk, UK
7 Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (FIOCRUZ), Pavilhão Leônidas Deane, Avenida Brasil 4365, CEP 21040-360, Rio de Janeiro, RJ, Brazil
8 Department of Genitourinary Medical Oncology, MD Anderson Cancer Center, 1515 Holcombe Blvd., Unit 1374 Medical Center, 77030 Houston, USA
Parasites & Vectors 2009, 2:35 doi:10.1186/1756-3305-2-35
Published: 31 July 2009Abstract
Background
Human infection by the pork tapeworm Taenia solium affects more than 50 million people worldwide, particularly in underdeveloped and developing countries. Cysticercosis which arises from larval encystation can be life threatening and difficult to treat. Here, we investigate for the first time the transcriptome of the clinically relevant cysticerci larval form.
Results
Using Expressed Sequence Tags (ESTs) produced by the ORESTES method, a total of 1,520 high quality ESTs were generated from 20 ORESTES cDNA mini-libraries and its analysis revealed fragments of genes with promising applications including 51 ESTs matching antigens previously described in other species, as well as 113 sequences representing proteins with potential extracellular localization, with obvious applications for immune-diagnosis or vaccine development.
Conclusion
The set of sequences described here will contribute to deciphering the expression profile of this important parasite and will be informative for the genome assembly and annotation, as well as for studies of intra- and inter-specific sequence variability. Genes of interest for developing new diagnostic and therapeutic tools are described and discussed.