Transcriptome analysis of Taenia solium cysticerci using Open Reading Frame ESTs (ORESTES)

doi:10.1186/1756-3305-2-35

Parasites & Vectors

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Almeida CR
Stoco PH
Wagner G
Sincero TC
Rotava G
Bayer-Santos E
Rodrigues JB
Sperandio MM
Maia AA
Ojopi EP
Zaha A
Ferreira HB
Tyler KM
Dávila AM
Grisard EC
Dias-Neto E

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Almeida CR
Stoco PH
Wagner G
Sincero TC
Rotava G
Bayer-Santos E
Rodrigues JB
Sperandio MM
Maia AA
Ojopi EP
Zaha A
Ferreira HB
Tyler KM
Dávila AM
Grisard EC
Dias-Neto E
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Transcriptome analysis of Taenia solium cysticerci using Open Reading Frame ESTs (ORESTES)

Carolina R Almeida¹ , Patricia H Stoco² , Glauber Wagner²^,3 , Thaís CM Sincero² , Gianinna Rotava² , Ethel Bayer-Santos² , Juliana B Rodrigues² , Maísa M Sperandio² , Antônio AM Maia⁴ , Elida PB Ojopi¹ , Arnaldo Zaha⁵ , Henrique B Ferreira⁵ , Kevin M Tyler⁶ , Alberto MR Dávila⁷ , Edmundo C Grisard²^,6 and Emmanuel Dias-Neto¹^,8

¹Laboratório de Neurociências (LIM27), Departamento e Instituto de Psiquiatria, Faculdade de Medicina da Universidade de São Paulo, Rua Dr. Ovídio Pires de Campos 785, CEP 05403-010, São Paulo, SP, Brazil

²Laboratórios de Protozoologia e de Bioinformática, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina (UFSC), Caixa postal 476, CEP 88040-970, Florianópolis, SC, Brazil

³Laboratório de Biologia Molecular e Bioinformática, Área das Ciências Biológicas e da Saúde, Universidade do Oeste de Santa Catarina (UNOESC), CEP 89600-000, Joaçaba, SC, Brazil

⁴Departamento de Ciências Básicas, Faculdade de Zootecnia e Engenharia de Alimentos. Universidade de São Paulo (USP), Av. Duque de Caxias Norte, 225, Centro, CEP 13630-000, Pirassununga, SP, Brazil

⁵Departamento de Biologia Molecular e Biotecnologia and Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves 9500, Prédio 4342131, Setor IV, Campus do Vale, CEP 91501-970, Porto Alegre, RS, Brazil

⁶Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia (UEA), NR4 7TJ, Norwich, Norfolk, UK

⁷Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (FIOCRUZ), Pavilhão Leônidas Deane, Avenida Brasil 4365, CEP 21040-360, Rio de Janeiro, RJ, Brazil

⁸Department of Genitourinary Medical Oncology, MD Anderson Cancer Center, 1515 Holcombe Blvd., Unit 1374 Medical Center, 77030 Houston, USA

author email corresponding author email

Parasites & Vectors 2009, 2:35doi:10.1186/1756-3305-2-35


Published:	31 July 2009

Abstract

Background

Human infection by the pork tapeworm Taenia solium affects more than 50 million people worldwide, particularly in underdeveloped and developing countries. Cysticercosis which arises from larval encystation can be life threatening and difficult to treat. Here, we investigate for the first time the transcriptome of the clinically relevant cysticerci larval form.

Results

Using Expressed Sequence Tags (ESTs) produced by the ORESTES method, a total of 1,520 high quality ESTs were generated from 20 ORESTES cDNA mini-libraries and its analysis revealed fragments of genes with promising applications including 51 ESTs matching antigens previously described in other species, as well as 113 sequences representing proteins with potential extracellular localization, with obvious applications for immune-diagnosis or vaccine development.

Conclusion

The set of sequences described here will contribute to deciphering the expression profile of this important parasite and will be informative for the genome assembly and annotation, as well as for studies of intra- and inter-specific sequence variability. Genes of interest for developing new diagnostic and therapeutic tools are described and discussed.