Loop-mediated isothermal amplification applied to filarial parasites detection in the mosquito vectors: Dirofilaria immitis as a study model
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* Corresponding authors: Shinya Fukumoto fukumoto@obihiro.ac.jp - Hirotaka Kanuka kanuka@obihiro.ac.jp
Parasites & Vectors 2009, 2:15 doi:10.1186/1756-3305-2-15
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BioMed Central: 3 citations
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Short report
Qing-Ming Kong, Shao-Hong Lu, Qun-Bo Tong, Di Lou, Rui Chen, Bin Zheng, Takashi Kumagai, Li-Yong Wen, Nobuo Ohta, Xiao-Nong Zhou Parasites & Vectors 2012, 5:2 (3 January 2012) This article is part of a collection on Aspects of parasitology in... We established a novel loop-mediated isothermal amplification targeting the 529 bp repeat element (529 bp-LAMP) to detect Toxoplasma gondii DNA in the blood samples of experimental mice infected with tachyzoites of the RH strain. Due to its simplicity, sensitivity and cost-effectiveness for common use, we suggest that this assay should be used as an early diagnostic tool for health control of toxoplasmosis. Image: Toxoplasma gondii tachyzoites cultured in HeLa cell monolayer.
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Short report
Rui Chen, QunBo Tong, Yi Zhang, Di Lou, QingMing Kong, Shan Lv, MingMing Zhuo, LiYong Wen, ShaoHong Lu Parasites & Vectors 2011, 4:204 (25 October 2011) This article is part of a collection on Aspects of parasitology in... Angiostrongylus cantonensis is a zoonotic parasite that causes eosinophilic meningitis in humans. The intermediate host Pomacea canaliculata plays an important role in the endemicity of Angiostrongyliasis cantonensis. This report describes a novel Loop-mediated isothermal amplification (LAMP) assay for the sensitive and specific detection of A. cantonensis in P. canaliculata. Image: Pomacea canaliculata, the intermediate host for Angiostrongylus cantonensis.
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Research
Zhi-Yong Tao, Hua-Yun Zhou, Hui Xia, Sui Xu, Han-Wu Zhu, Richard L Culleton, Eun-Taek Han, Feng Lu, Qiang Fang, Ya-Ping Gu, Yao-Bao Liu, Guo-Ding Zhu, Wei-Ming Wang, Ju-Lin Li, Jun Cao, Qi Gao Parasites & Vectors 2011, 4:115 (21 June 2011) This article is part of a collection on Aspects of parasitology in... This study demonstrated that a novel visualized loop-mediated isothermal amplification (LAMP) assay for Plasmodium vivax detection, was accomplished using a microcrystalline wax-dye capsule. Image: Visualized LAMP results by SYBR-Green I contained in microcrystalline wax capsules.
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