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Gene specific primers used for RT-PCR expression profiling of I. ricinus peptidases |
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| peptidase |
forward primer |
reverse primer |
annealing temperature |
product length |
|
|
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| IrAE |
5'TCGGTGACGCTGAGAAGACTGAA3' |
5'TAGATTATGCCCGATGACTGTTGG3' |
55°C |
239 bp |
| IrCB |
5'CGACGGCGGATACCCGGCGGCAGC3' |
5'CCGAGTACGTCACCGCTGTGGTGC3' |
60°C |
360 bp |
| IrCL |
5'GTCACGCCCGTCAAGAACC3' |
5'TGGCATCAATGGCAACAGAGA3' |
62°C |
382 bp |
| IrCC |
5'CCACCCGGGACCCCTACAACACC3' |
5'GGAAGCCGCCCTCGCAACC3' |
68°C |
447 bp |
| IrCD |
5'GGACCCCGCCCCAAGACT3' |
5'CGATGCCGCCGAACAGGA3' |
61°C |
459 bp |
Sojka et al. Parasites & Vectors 2008 1:7 doi:10.1186/1756-3305-1-7 |
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