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Degenerate PCR primers used for identification of genes encoding gut-associated peptidases of I. ricinus |
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| peptidase |
cathepsin B |
cathepsin L |
cathepsin C |
cathepsin D |
|
|
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| forward primer |
5'CKTGYGGDTCTTGTTGGG 3' |
5'CAGTGCGGVTCCTGCTGGGC3' |
5'GTCGACACKCCYGCBAACTGCAC3' |
5'TCACCCCAGCCKNCGTTCCA3' |
| reverse primer |
5'GGCTCCTCTAAYYTBTGGGT 3' |
5'GGCGATTCCGCACTGGTTGT3' |
5'CCRTAGWAACCWCCMACATA3' |
5'GAACACGTCNCCBARDATCCA3' |
| Sm* GenBank |
CAC85211 |
CAA83538 |
CAA83543 |
AAB63442 |
| Sm* forward domain |
117SSCGSCWA124 |
123LCGSCWA129 |
20ADTPANC26 |
85GSSNLWV91 |
| Sm* reverse domain |
313WNSDWGD319 |
304NMCGIA309 |
337YIGGYY342 |
355WILGDIF361 |
| annealing temperature |
55°C |
53°C |
62°C |
53°C |
| product length |
608 bp |
554 bp |
998 bp |
855 bp |
| sequenced clones |
12 |
8 |
8 |
8 |
| isoforms |
2 |
1 |
1 |
1 |
|
*- orthologues in Schistosoma mansoni | ||||
Sojka et al. Parasites & Vectors 2008 1:7 doi:10.1186/1756-3305-1-7 |
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