Additional file 1.
A single adult female living specimen of Heligmosomoides bakeri was mounted on a microscope slide in Hanks's saline and sandwiched beneath a glass coverslip supported on petroleum jelly. The worm was imaged using a Zeiss Axiovert 135TV inverted microscope and photographed using a Scion CFW 1310 M digital camera. A solution of 25 μM papain was introduced below the coverslip and images were captured on a PC using Streampix III time-lapse software at a frame rate of approximately 1 image every 3 seconds for 30 minutes. The video file was edited and exported as an mpeg running at 10 times the original speed. The file is titled "H. bakeri female papain.mpg" and initially shows the worm freely moving in the papain solution. After the animal forms a helical coil, a lesion appears on the left of the worm. This is followed by rupture of the worm and loss of the viscera through the rupture leading to the death of the parasite.
Format: MPG Size: 2.3MB Download file
Behnke et al. Parasites & Vectors 2008 1:29 doi:10.1186/1756-3305-1-29